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KMID : 0378019820250100079
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New Medical Journal
1982 Volume.25 No. 10 p.79 ~ p.84
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Effect of sensitizing temperature and time on helper T lymphocytes quantitation
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Abstract
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Peripheal blood from 34 normal healthy Korean subjects was examined for the quantita= tion of helper T lymphocytes. We counted the helper T lymphocytes by using the conventional double rosette mothod and modified sensitizing method. It is supposed that the factor
affecting the number of helper T lymphocytes is sensitizing temperature and time.
The procedures for the sensitizing temperature and time during the sensitization of ox RBC with anti ox RBC rabbit Ig M in the conventional double rosette method was done for 2 hours at room temperature, while in the modified method, for 30 mintes at 37°C water bath. We compared statistically the resulting data by using the conventional and modified sensitizing method. The results are as follows.
1. Means of helper T lymphocytes were 28.6±7.1% in the coventional double rosette mothod.
2. Means of helper T lymphocytes were 42.2±8.000 in the modified sensitizing method. 3. This difference is statistically significant (p<0.005).
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